Tesi etd-09062023-162424
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Type of thesis
Dottorato
Author
MOTA DA SILVA, EDUARDA
URN
etd-09062023-162424
Title
Biochemical and biohumoral studies of bone regeneration and remodelling after implantation of magnesium pin
Scientific disciplinary sector
MED/33
Course
Istituto di Scienze della Vita - PHD IN MEDICINA TRASLAZIONALE
Committee
relatore Prof. MENICHETTI, LUCA
Keywords
- biodegradable implant
- immunological response
- magnesium alloy
- systemic biomarkers
Exam session start date
22/04/2024;
Availability
completa
Abstract
Magnesium degradable implants, notable for their mechanical and osteogenic properties, are<br>ideal for temporary orthopedic applications but face limited clinical use due to inadequate<br>follow-up methods for assessing implant osseointegration and tissue regeneration. This study<br>assessed the potential of circulatory systemic biomarkers to monitor inflammation and bone<br>regeneration following the bilateral implantation of Mg-alloys in rat’s femurs.<br>Sixteen biomarkers of inflammation and bone regeneration were measured from plasma<br>samples collected at multiple time-points up to 90 days after surgery. All animals (Mg-alloy<br>group, Titanium group and SHAM -no-critical bone defect group) were monitored for pin<br>placement and bone regeneration using computed tomography.<br>Noteworthy findings included the higher concentration of OPG, DKK1, VEGF, and KIM-1 in<br>SHAM group compared to implanted animals. The Mg-alloy group (WE43) showed lower<br>concentrations of OPG and VEGF compared to titanium group on days 7 and 28. Histological<br>analysis showed progressive bone regeneration around both types of implants, but WE43<br>degradation promoted a delayed regenerative process in respect to titanium. A notable finding<br>was the low concentration of FGF23 and the high concentration of IL10 on day 28 in the WE43<br>group associated with a thicker intramedullary corrosion layer assessed through SEM/EDX<br>analysis of bone-implant interface. Furthermore, the trace accumulation of WE43 degradation<br>products (Y,REE) showed variable concentrations over time in the liver, spleen and kidney.<br>This study provides valuable insights into the behavior of different implant materials in vivo,<br>offering important implications on the safety of Mg-alloy implants and exploring the utility of<br>systemic biomarkers as a follow-up technique.
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