Tesi etd-03302021-191945
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Tipo di tesi
Dottorato
Autore
SODINI, MIRKO
URN
etd-03302021-191945
Titolo
Olea europaea and salinity: understanding ions exclusion and compartmentalization
Settore scientifico disciplinare
AGR/03
Corso di studi
Istituto di Scienze della Vita - AGROBIOSCIENCES
Commissione
relatore Prof. SEBASTIANI, LUCA
Parole chiave
- olive tree
- salt stress
- sodium
- SOS1
- ATPase
- NHX
Data inizio appello
09/07/2021;
Disponibilità
completa
Riassunto analitico
Saline stress on olive trees has been studied since the early 90s. Although the resistance of the olive tree to salt depends on the exclusion of Na+ from the leaf part, little is known about the mechanisms of Na+ mobilization. The purpose of this work is to broaden the knowledge of sodium compartmentation and exclusion in the olive tree. We chose to investigate the trans membrane pumps responsible for sodium management in cells: SOS1, PM-ATPase, NHX and V-ATPase. These pumps have been extensively studied in many plant species, but poorly in olive trees. For this reason, the firsts chapter is the development of enzymatic protocols, primers selection and sequencing of our target genes, with the aim of obtaining tools to study the role of these membrane pumps in olive trees. In the second chapter, we studied the genes that encode the target membrane pumps in the roots of a susceptible cultivar. The aim was to monitor the early stages of salt stress and find possible involvement of these genes in the sodium translocation. In the third chapter we studied the same genes in the leaves of two cultivars with opposite tolerance. The purpose was to test the hypothesis of a different expression of these genes in response to the sodium translocation. The data collected in our works were analyzed with an innovative approach compared to previous studies. We built more complex statistical models, which revealed interesting relationships between our data. The combination of all these tools allowed us to add new information to understand the translocation of sodium in the Olea europaea L. species.
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