Tesi etd-04142023-113941
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Tipo di tesi
Dottorato
Autore
VICARIO, GIULIA
URN
etd-04142023-113941
Titolo
From olive fruit to olive oil: relation between olive abiotic stress response as well as agricultural practices and quality of fruit and oil
Settore scientifico disciplinare
AGR/03
Corso di studi
Istituto di Scienze della Vita - PHD IN AGROBIOSCIENZE
Commissione
Nessun commissario trovato
Parole chiave
- diclofenac
- irrigation
- nickel
- olive
- olive oil
- soil tillage
Data inizio appello
20/07/2023;
Disponibilità
parziale
Riassunto analitico
In order to both obtain high quality olive products and maintain optimal plant growth and physiology in a water scarcity scenario, a lot of efforts have been done to understand and characterize the response of olive tree to soil and water management. The purpose of this thesis work is to broaden the knowledge in this field, mainly focusing on two aspects. With respect to the first aspect, we would like to better understand the impact of both the reduction of the water supply and the soil tillage on the quality of the olive fruits and of the produced olive oil (Annex I and Annex II). Notably, we applied powerful techniques that allowed us also to quantify trace compounds that are not usually described in fruits and oils. In the first part of the thesis (Annex I), we described the characterization of the olive fruits phenolic compounds through UHPLC-MS/MS as well as the minerals reported in the fruit pulp. Having observed interesting differences in olive fruits from the first work, in the second part of this thesis (Annex II), we mainly focused on the oil quality, especially on secoiridoids and pigments characterization through innovative spectroscopic methods (Nuclear Magnetic Resonance (NMR) and Near UV-Vis spectroscopy). The methods developed for phenolic compounds and minerals analysis were then employed in later works (Annex III and IV). The second aspect we would like to explore is the impact of emerging contaminants (i.e., Diclofenac) and heavy metals (i.e., Nickel) on olive tree (Annex III and Annex IV, respectively). The knowledge in this specific field is growing, but few is known about olive tree. For this reason, for both the third and the fourth part of the thesis, we decided to broad our focus including not only the characterization of the olive fruit but also the physiological responses of the whole olive tree. In addition, we also used molecular biology tools (i.e., gene expression analysis) to better understand the olive fruit response to contaminants exposure.
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