DTA

Digital Theses Archive

 

Tesi etd-05072020-155740

Type of thesis
Corsi integrativi di II livello
Author
GIANNINI, CATERINA
URN
etd-05072020-155740
Title
Engineering of Ustilago maydis: a new method to identify novel significant effectors interaction targets at the biotrophic interface
Structure
Cl. Sc. Sperimentali - Agraria
Course
SCIENZE AGRARIE E BIOTECNOLOGIE - SCIENZE AGRARIE E BIOTECNOLOGIE
Committee
Tutor Prof. PE', MARIO ENRICO
Relatore Prof.ssa PUCCIARIELLO, CHIARA
Presidente Prof. SEBASTIANI, LUCA
Membro Dott. DELL'ACQUA, MATTEO
Membro Dott. ZUCCOLO, ANDREA
Membro Prof.ssa MENSUALI, ANNA
Keywords
  • Ustilago maydis effectors plant immunitychitinase
Exam session start date
;
Availability
parziale
Abstract
In every host-pathogen system, a successful infection relies on the pathogen to overcome the host defense mechanisms.<br>The biotrophic pathogenic fungus Ustilago maydis requires a living host for nutrient uptake, to complete its life cycle. In particular, it infects maize (Zea mays), causing symptoms on all aerial parts of the plant, resulting in the corn smut disease. <br>To succeed in the infection, preventing plant immunity responses, small molecules, named effectors are secreted by the fungal pathogen in the apoplast, acting in this space, at the so-called biotrophic interface, or inside the plant cell, after being translocated. <br>For example, effectors can fulfill their function by targeting plant apoplastic proteins, secreted upon the perception of the pathogen, hampering their antifungal activity.<br>This study aims to develop a new method to characterize significant plant protein - effector protein interactions, possibly occurring in maize apoplast during Ustilago maydis infection, exploiting engineered Ustilago maydis strains in axenic culture.<br>
Files