DTA

Corso Ordinario Secondo Livello

GRANATA, ELEONORA

etd-05232024-102200

Orchard floor management affects the diversity and composition of arbuscular mycorrhizal fungi in a Mediterranean olive orchard over two crop seasons

Classe Scienze Sperimentali

SCIENZE AGRARIE E BIOTECNOLOGIE - SCIENZE AGRARIE E BIOTECNOLOGIE

Tutor Prof.ssa PUCCIARIELLO, CHIARA
Presidente Prof. TONUTTI, PIETRO
Membro Dott. CAPRONI, LEONARDO
Relatore Prof.ssa TURRINI, ALESSANDRA
Relatore Prof.ssa AGNOLUCCI, MONICA
Membro Dott.ssa BARTOLINI, SUSANNA
Membro Prof. SEBASTIANI, LUCA

• Arbuscular mycorrhizal fungi
• cover crops
• Illumina Miseq
• metabarcoding
• natural grass cover
• olive orchard
• virtual taxa

18/06/2024;

parziale

The olive tree (Olea europea L.) is a perennial and evergreen plant species representing one of the most important woody crops cultivated in the Mediterranean area. Arbuscular mycorrhizal fungi (AMF) are beneficial soil microorganisms that can form mutualistic symbiosis with most plant species, including olive trees. Such characteristics are fundamental in agroecology and can accompany sustainable agronomic practices to promote soil health and fertility. The thesis aimed at analyzing the AMF communities' biodiversity in soil samples of an experimental olive orchard subjected to two treatments: natural grass cover and cover crops. 231 unique sequences (ASV) have been found via bioinformatic pipeline and grouped into 31 virtual taxa (VTX). Among the diversity indices analyzed, the richness was influenced by treatment and time, with lower values for the cover crops treatment rather than natural grass cover in the first year. The PERMANOVA analysis showed significant differences in the structure of the AMF communities between the two years. The results showed that natural grass cover positively affects the structure of AMF communities in the soil.

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