Tesi etd-07122023-121048
Link copiato negli appunti
Type of thesis
Dottorato
Author
GÓMEZ ÁLVAREZ, EVA MARÍA
URN
etd-07122023-121048
Title
Germination of barley after submergence: a genetics and a microbiome approach
Scientific disciplinary sector
BIO/04
Course
Istituto di Scienze della Vita - PH.D. IN AGROBIODIVERSITY
Committee
relatore PUCCIARIELLO, CHIARA
Keywords
- Barley
- germination
- microbiome
- submergence
Exam session start date
18/12/2023;
Availability
parziale
Abstract
I studied barley germination tolerance to short submergence stress through genetic and microbiome approaches. Barley is the most sensitive cereal to submergence, especially during germination. In the past four years, I have phenotyped the WHEALBI barley collection for the trait Submergence Tolerance Index (STI), identifying tolerant and sensitive genotypes to short submergence periods. Through a Genome Wide Association Studies, I was able to identify a laccase as the main responsible of the tolerance variation under the stress. Hence, I show that the differential expression of a specific gene during seed maturation affects the tolerance variation to an abiotic stress during germination. Although differential laccase haplotypes explain the different phenotypes, different seed microbiota can also contribute. Thus, I performed the analysis of a metagenomic 16S rRNA gene amplicon sequencing on tolerant and sensitive barley grains. Obtained results suggest the presence of a dissimilar microbiome availability in tolerant versus sensitive accessions of barley and support the hypothesis that this difference may be responsible of some activities involved in barley germination after submergence. Overall, this works gives an overview in the response of barley to short submergence periods during germination, from the genetics to the microbiome.
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