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Tesi etd-07122023-141448

Type of thesis
Dottorato
Author
GERMANO, ROCCO PIERPAOLO
URN
etd-07122023-141448
Title
Biodiversity as a Source for High-Throughput Screening
Scientific disciplinary sector
BIO/04
Course
Istituto di Scienze della Vita - PH.D. IN AGROBIODIVERSITY
Committee
relatore Prof. PERATA, PIERDOMENICO
Keywords
  • Screening of bioactive molecules
Exam session start date
04/12/2023;
Availability
parziale
Abstract
The aim of the project is to develop a rapid screening system of chemical library to identify new bioactive substances capable of significantly influencing a biological process, therefore a specific physiological trait in the treated plants, including the response to specific exogenous stress.<br>Given the significant number of molecules object of the screening, the development of a rapid monitoring system was essential. For this purpose, it was chosen to use Arabidopsis as model plant to grow and treat on a High Throughput System platform. <br>In the first year, the activities have mainly concerned the experimental set-up for this high throughput screening system.<br>Once the system has been optimized, then defined the stage of plant development as well as the suitable concentrations of treatment, the activities of the second year focused on:<br><br>• Bioassay to select the molecules, among those tested, capable of inducing a phenotypic effect on treated plants, detectable in the significant variation of one of the biometric parameters taken into consideration;<br><br>• Categorization of tested molecules based on the specific resulted action;<br><br>• Investigation concerning the mechanism of action.<br>
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