Tesi etd-09062023-162424
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Tipo di tesi
Dottorato
Autore
MOTA DA SILVA, EDUARDA
URN
etd-09062023-162424
Titolo
Biochemical and biohumoral studies of bone regeneration and remodelling after implantation of magnesium pin
Settore scientifico disciplinare
MED/33
Corso di studi
Istituto di Scienze della Vita - PHD IN MEDICINA TRASLAZIONALE
Commissione
relatore Prof. MENICHETTI, LUCA
Parole chiave
- biodegradable implant
- immunological response
- magnesium alloy
- systemic biomarkers
Data inizio appello
22/04/2024;
Disponibilità
completa
Riassunto analitico
Magnesium degradable implants, notable for their mechanical and osteogenic properties, are
ideal for temporary orthopedic applications but face limited clinical use due to inadequate
follow-up methods for assessing implant osseointegration and tissue regeneration. This study
assessed the potential of circulatory systemic biomarkers to monitor inflammation and bone
regeneration following the bilateral implantation of Mg-alloys in rat’s femurs.
Sixteen biomarkers of inflammation and bone regeneration were measured from plasma
samples collected at multiple time-points up to 90 days after surgery. All animals (Mg-alloy
group, Titanium group and SHAM -no-critical bone defect group) were monitored for pin
placement and bone regeneration using computed tomography.
Noteworthy findings included the higher concentration of OPG, DKK1, VEGF, and KIM-1 in
SHAM group compared to implanted animals. The Mg-alloy group (WE43) showed lower
concentrations of OPG and VEGF compared to titanium group on days 7 and 28. Histological
analysis showed progressive bone regeneration around both types of implants, but WE43
degradation promoted a delayed regenerative process in respect to titanium. A notable finding
was the low concentration of FGF23 and the high concentration of IL10 on day 28 in the WE43
group associated with a thicker intramedullary corrosion layer assessed through SEM/EDX
analysis of bone-implant interface. Furthermore, the trace accumulation of WE43 degradation
products (Y,REE) showed variable concentrations over time in the liver, spleen and kidney.
This study provides valuable insights into the behavior of different implant materials in vivo,
offering important implications on the safety of Mg-alloy implants and exploring the utility of
systemic biomarkers as a follow-up technique.
ideal for temporary orthopedic applications but face limited clinical use due to inadequate
follow-up methods for assessing implant osseointegration and tissue regeneration. This study
assessed the potential of circulatory systemic biomarkers to monitor inflammation and bone
regeneration following the bilateral implantation of Mg-alloys in rat’s femurs.
Sixteen biomarkers of inflammation and bone regeneration were measured from plasma
samples collected at multiple time-points up to 90 days after surgery. All animals (Mg-alloy
group, Titanium group and SHAM -no-critical bone defect group) were monitored for pin
placement and bone regeneration using computed tomography.
Noteworthy findings included the higher concentration of OPG, DKK1, VEGF, and KIM-1 in
SHAM group compared to implanted animals. The Mg-alloy group (WE43) showed lower
concentrations of OPG and VEGF compared to titanium group on days 7 and 28. Histological
analysis showed progressive bone regeneration around both types of implants, but WE43
degradation promoted a delayed regenerative process in respect to titanium. A notable finding
was the low concentration of FGF23 and the high concentration of IL10 on day 28 in the WE43
group associated with a thicker intramedullary corrosion layer assessed through SEM/EDX
analysis of bone-implant interface. Furthermore, the trace accumulation of WE43 degradation
products (Y,REE) showed variable concentrations over time in the liver, spleen and kidney.
This study provides valuable insights into the behavior of different implant materials in vivo,
offering important implications on the safety of Mg-alloy implants and exploring the utility of
systemic biomarkers as a follow-up technique.
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