Tesi etd-10022025-092441
Link copiato negli appunti
Tipo di tesi
Corso Ordinario Secondo Livello
Autore
MALTONI, TOMMASO
URN
etd-10022025-092441
Titolo
Transcriptomic analysis of an Arabidopsis thaliana fermentative mutant during de novo shoot regeneration
Struttura
Classe Scienze Sperimentali
Corso di studi
SCIENZE AGRARIE E BIOTECNOLOGIE - SCIENZE AGRARIE E BIOTECNOLOGIE
Commissione
Tutor Prof.ssa PUCCIARIELLO, CHIARA
Membro Dott. CAPRONI, LEONARDO
Presidente Prof. BARBERI, PAOLO
Membro Dott. CARLESI, STEFANO
Membro Prof.ssa MOONEN, ANNA CAMILLA
Relatore Prof.ssa giuntoli, beatrice
Membro Dott. CAPRONI, LEONARDO
Presidente Prof. BARBERI, PAOLO
Membro Dott. CARLESI, STEFANO
Membro Prof.ssa MOONEN, ANNA CAMILLA
Relatore Prof.ssa giuntoli, beatrice
Parole chiave
- Arabidopsis thaliana
- callus
- cell wall
- ERF109
- hypoxia
- metabolism
- organogenesis
- plants
- pyruvate
- regeneration
- regeneration
- trascription
Data inizio appello
12/12/2025;
Disponibilità
parziale
Riassunto analitico
"In plants hypoxia, i.e. the scarce availability of oxygen in tissues, is a common condition. It can be caused by external stressful events, such as floods, or be present physiologically, in tissues in which the diffusion of oxygen is limited, such as in meristems and fruits. This also happens during the in vitro regeneration of plants, when cell proliferation leads to the formation of structures called calli, inside which oxygen is scarce. When cells do not have access to enough oxygen, they cannot use the electron transport chain to regenerate oxidized cofactors; therefore, they use alternative metabolic pathways such as lactic fermentation, alcoholic fermentation, and non-circular citric acid pathways to allow glycolysis to continue. In this work, we analyzed plants of the species Arabidopsis thaliana mutant for genes fundamental for these metabolic pathways (individually or in higher-order combinations of mutations), namely lactate dehydrogenase (LDH), the two hypoxia-inducible isoforms of pyruvate decarboxylase (PDC1/2) and alanine aminotransferases (ALTAA1/2). We analyzed the in vitro regeneration of these mutants, evaluating the time required for the formation of sprouts. We analyzed differences in gene expression between mutant and wild-type plants at different times of regeneration using the RNA-seq technique. The work revealed for the first time the differential contribution of pyruvate fermentative pathways in wounding response and cell wall modification during de novo organogenesis."
File
| Nome file | Dimensione |
|---|---|
Ci sono 1 file riservati su richiesta dell'autore. |
|