Tesi etd-10192024-175043
Link copiato negli appunti
Tipo di tesi
Corso Ordinario Secondo Livello
Autore
GRAVILI, IRENE
URN
etd-10192024-175043
Titolo
Induction, stabilization, and characterization of suspension cell cultures of Ocimum basilicum L.
Struttura
Classe Scienze Sperimentali
Corso di studi
SCIENZE AGRARIE E BIOTECNOLOGIE - SCIENZE AGRARIE E BIOTECNOLOGIE
Commissione
Tutor Prof.ssa PUCCIARIELLO, CHIARA
Relatore Prof.ssa MENSUALI, ANNA
Membro Dott.ssa FRANCINI, ALESSANDRA
Membro Dott. CARLESI, STEFANO
Membro Prof. DELL'ACQUA, MATTEO
Membro Prof. PERATA, PIERDOMENICO
Membro Dott. ROSSETTO, RUDY
Relatore Dott.ssa BURBI, SARA
Relatore Prof.ssa MENSUALI, ANNA
Membro Dott.ssa FRANCINI, ALESSANDRA
Membro Dott. CARLESI, STEFANO
Membro Prof. DELL'ACQUA, MATTEO
Membro Prof. PERATA, PIERDOMENICO
Membro Dott. ROSSETTO, RUDY
Relatore Dott.ssa BURBI, SARA
Parole chiave
- cellular agriculture
- Novel Foods
- Ocimum basilicum L.
- plant cell suspension culture
Data inizio appello
10/12/2024;
Disponibilità
completa
Riassunto analitico
Climate change and population growth pose new challenges in the field of Food Security, leading to a fundamental critique of traditional food production techniques. Hence, it is fundamental to develop innovative practices to reach sustainability in the Food Chain. At the European level, these new practices and their products fall under the Novel Food regulation. In the highly debated context of Novel Food, the project presented hereby tries to explore the alternative of Cellular Agriculture as a production system.
The aim is to demonstrate in the model species Ocimum basilicum L. that plant cell suspension cultures represent a valid and safe alternative to traditional food on a small-scale. Despite the great potential offered by plant cell cultures, many aspects still need to be resolved before this new type of food can enter the market. In particular, our focus was on minimizing or completely replacing Plant Growth Regulators (PGRs). Cell suspension cultures were obtained from callus cultures, induced from leaf explants by testing different PGR concentrations and compositions. Subsequently, the influence of PGR doses on cell growth was evaluated, along with the total polyphenol production and the antioxidant capacity of the molecules using Folin-Ciocâlteu and DPPH assays. Starting from callus cells, suspension cultures were initiated with decreasing or absent PGR concentrations. The cultures were characterized for growth, as previously described. Then, through HPLC-MS/MS, the metabolomic profile of hormones and polyphenols content was defined both in the cells and in the exhausted media of cultures with decreasing or absent PGR. The analysis showed that higher PGR concentrations and/or mixed PGR formulations did not have a significantly positive effect on callus induction and growth on solid media. In cell cultures, the most suitable growth dynamic to sustain growth in bioreactor was demonstrated in the lowest PGR condition. Coherently, biochemical characterization suggested a higher capacity for polyphenol accumulation in cells grown under low synthetic auxin concentration or in its absence. These cells, both with and without PGR, showed a great anti-oxidant capacity.
The aim is to demonstrate in the model species Ocimum basilicum L. that plant cell suspension cultures represent a valid and safe alternative to traditional food on a small-scale. Despite the great potential offered by plant cell cultures, many aspects still need to be resolved before this new type of food can enter the market. In particular, our focus was on minimizing or completely replacing Plant Growth Regulators (PGRs). Cell suspension cultures were obtained from callus cultures, induced from leaf explants by testing different PGR concentrations and compositions. Subsequently, the influence of PGR doses on cell growth was evaluated, along with the total polyphenol production and the antioxidant capacity of the molecules using Folin-Ciocâlteu and DPPH assays. Starting from callus cells, suspension cultures were initiated with decreasing or absent PGR concentrations. The cultures were characterized for growth, as previously described. Then, through HPLC-MS/MS, the metabolomic profile of hormones and polyphenols content was defined both in the cells and in the exhausted media of cultures with decreasing or absent PGR. The analysis showed that higher PGR concentrations and/or mixed PGR formulations did not have a significantly positive effect on callus induction and growth on solid media. In cell cultures, the most suitable growth dynamic to sustain growth in bioreactor was demonstrated in the lowest PGR condition. Coherently, biochemical characterization suggested a higher capacity for polyphenol accumulation in cells grown under low synthetic auxin concentration or in its absence. These cells, both with and without PGR, showed a great anti-oxidant capacity.
File
| Nome file | Dimensione |
|---|---|
| TesinaIG.pdf | 1.65 Mb |
Contatta l'autore |
|