Tesi etd-11192025-143807
Link copiato negli appunti
Tipo di tesi
Corso Ordinario Secondo Livello
Autore
FARANO, ANNA CARLA
URN
etd-11192025-143807
Titolo
Cross-kingdom RNA interference in plants
Struttura
Classe Scienze Sperimentali
Corso di studi
SCIENZE AGRARIE E BIOTECNOLOGIE - SCIENZE AGRARIE E BIOTECNOLOGIE
Commissione
Tutor Prof. DELL'ACQUA, MATTEO
Presidente Prof. BARBERI, PAOLO
Membro Prof.ssa MOONEN, ANNA CAMILLA
Membro Prof.ssa PUCCIARIELLO, CHIARA
Membro Dott. CAPRONI, LEONARDO
Membro Dott. CARLESI, STEFANO
Relatore Prof.ssa leonetti, paola
Presidente Prof. BARBERI, PAOLO
Membro Prof.ssa MOONEN, ANNA CAMILLA
Membro Prof.ssa PUCCIARIELLO, CHIARA
Membro Dott. CAPRONI, LEONARDO
Membro Dott. CARLESI, STEFANO
Relatore Prof.ssa leonetti, paola
Parole chiave
- Cross-kingdom RNA interference
- Small RNAs
- MicroRNAs
- Plant-pathogen interactions
- Gene silencing
- Extracellular vesicles
- RNA-based plant defense
Data inizio appello
11/12/2025;
Disponibilità
completa
Riassunto analitico
Cross-kingdom RNA interference (ckRNAi) is transforming how we understand communication between plants and the organisms that interact with them. In this thesis, I explore how small RNAs move between kingdoms, how they shape plant immunity and pathogen virulence, and how we can study these pathways to develop crop protection technologies.
I begin by comparing the RNAi machinery across different kingdoms and how RNA molecules travel between different species to cross physical and taxonomic boundaries.
Next, I present key case studies where plants and their interacting organisms exchange RNA to influence each other’s gene expression, plant defense, pathogen virulence, and even symbiotic relationships. Building on this, I discuss RNA-based crop protection strategies as host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS) as practical applications of ckRNA.
Finally, I address the major challenges facing the field, including experimental difficulties and bioinformatics uncertainties. Altogether, this work brings together mechanistic and applied perspectives to show how cross-kingdom RNA exchange works in nature and how it can be used to develop more precise, sustainable plant protection strategies.
Altogether, ckRNAi is expanding quickly and shows great promise, yet key mechanisms remain unclear and several proposed interactions still lack definitive proof. Therefore, careful interpretation and deeper investigation are needed to unravel the full significance of these interactions and to develop RNAi-based solutions that can truly drive agricultural innovation.
I begin by comparing the RNAi machinery across different kingdoms and how RNA molecules travel between different species to cross physical and taxonomic boundaries.
Next, I present key case studies where plants and their interacting organisms exchange RNA to influence each other’s gene expression, plant defense, pathogen virulence, and even symbiotic relationships. Building on this, I discuss RNA-based crop protection strategies as host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS) as practical applications of ckRNA.
Finally, I address the major challenges facing the field, including experimental difficulties and bioinformatics uncertainties. Altogether, this work brings together mechanistic and applied perspectives to show how cross-kingdom RNA exchange works in nature and how it can be used to develop more precise, sustainable plant protection strategies.
Altogether, ckRNAi is expanding quickly and shows great promise, yet key mechanisms remain unclear and several proposed interactions still lack definitive proof. Therefore, careful interpretation and deeper investigation are needed to unravel the full significance of these interactions and to develop RNAi-based solutions that can truly drive agricultural innovation.
File
| Nome file | Dimensione |
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| ckRNAi_5.pdf | 9.08 Mb |
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