Tesi etd-12282017-190420
Link copiato negli appunti
Tipo di tesi
Perfezionamento
Autore
PIGNATELLI, DANIELA
URN
etd-12282017-190420
Titolo
Synthesis and functionalization of innovative nanomaterials for biomedical applications
Settore scientifico disciplinare
ING-IND/34
Corso di studi
INGEGNERIA - Biorobotics
Commissione
relatore DARIO, PAOLO
Parole chiave
- Free-standing
- Hydroxyapatite
- Nanofilms
- Nanomaterials
- Nanoparticles
- Parole chiave: Cells
- Plasma process
- Selenium
- Silver
- Tissue engineering.
Data inizio appello
11/12/2018;
Disponibilità
completa
Riassunto analitico
1 Introduction 6
1.1 Thesis outline 9
2 Free-standing Nanofilms containing molecules of Vancomycin produced by atmospheric pressure plasma process 13
2.1 Introduction 13
2.1.1 Uses of Vancomycin in biomedical fields 13
2.1.2 Atmospheric pressure plasma process to produce bio-composite coating 16
2.2 Materials and Methods 21
2.2.1 Spin coating deposition parameters to produce sacrificial layer for nanofilm release in water 21
2.2.2 Plasma processes reactor setup 23
2.2.3 Experimental plasma parameters 24
2.2.4 Release of free-standing nanofilms in water 25
2.2.5 NFs Characterization (FT-IR, profilometer, SEM analysis, NFs release test) 26
2.3 Results and discussion 28
2.3.1 Study of delamination, morphology and chemical properties. 28
2.3.2 Study of Vancomycin release in water 31
2.4 Conclusion 31
Chapter 3 32
3 NFs embedding silver nanoparticles and NFs with unfouling and cell-adhesive properties produced by low pressure plasma processes 32
3.1 Introduction 32
3.1.1 Nanofilm embedding silver nanoparticles for antibacterial applications 32
Nanofilm with unfouling cell-adhesive properties in biomedical field produced by PE-CVD 37
3.2 Materials and Methods 40
3.2.2 Reactor setup 41
3.2.3 Experimental Plasma Parameters 42
Characterization of NFs 43
3.3 Results and discussion 44
3.3.1 Study of morphology and chemical properties. 44
3.4 Conclusion 50
4 Low pressure plasma enhanced chemical vapour deposition of chemically different films for biomedical applications 52
4.1 Hydrophobic Teflon like films 54
4.1.1 Introduction 54
4.1.2 Materials and Methods 57
4.1.3 Results and discussion 62
4.2 Films with a different density of COOH on their surface 71
4.2.1 Introduction 71
4.2.2 Materials and Methods 73
4.2.3 Results and discussion 75
4.2.4 Conclusions 90
5 Biological characterization of films produced by plasma processes 92
5.1 Introduction 92
5.1.1Primary cells and Immortal Cell line Cultures 92
5.1.2 In vitro Study of Cells: Evaluation of Adhesion and Viability 97
5.2 Materials and Methods 101
5.2.1 Saos2 cell line 101
5.2.2 Coomassie Blue staning assay 102
5.2.3 MTT test 102
5.3 Results and discussion 104
5.3.1.2. Study of the effect on Saos2 cells of hydrophilic surfaces produced by plasma deposition: effect of CO2 in the feed gas mixture 107
5.4 Conclusion 116
6 Synthesis of Hydroxyapatite Nanoparticles doped with different amount of selenium for tissue engineering applications 117
6.1 Introduction 117
6.1.1 Role of selenium in the biomedical field 117
6.1.2 Synthesis and functionalization of hydroxyapatite nanoparticles 123
6.2 Materials and Methods 130
6.2.1 Synthesis of hydroxyapatite nanoparticles (npHA) 130
6.2.2 Synthesis of hydroxyapatite nanoparticles doped with different percent of selenium (npHA_Se) 131
6.2.3 Chemical-physical characterization of nanoparticles: TEM, SEM, XRD 133
6.2.4 Testing for nanoparticles citotoxicity 135
6.3 Results and discussion 138
6.3.1 Effect of different amount of selenium on morphology, cristalline structure and chemical composition of npHA_Se 138
6.3.2 Effect of hydroxyapatite nanoparticles doped with different percent of selenium on two different cell types: Saos2 and BMSC. 141
6.4 Conclusion 146
References 147
1.1 Thesis outline 9
2 Free-standing Nanofilms containing molecules of Vancomycin produced by atmospheric pressure plasma process 13
2.1 Introduction 13
2.1.1 Uses of Vancomycin in biomedical fields 13
2.1.2 Atmospheric pressure plasma process to produce bio-composite coating 16
2.2 Materials and Methods 21
2.2.1 Spin coating deposition parameters to produce sacrificial layer for nanofilm release in water 21
2.2.2 Plasma processes reactor setup 23
2.2.3 Experimental plasma parameters 24
2.2.4 Release of free-standing nanofilms in water 25
2.2.5 NFs Characterization (FT-IR, profilometer, SEM analysis, NFs release test) 26
2.3 Results and discussion 28
2.3.1 Study of delamination, morphology and chemical properties. 28
2.3.2 Study of Vancomycin release in water 31
2.4 Conclusion 31
Chapter 3 32
3 NFs embedding silver nanoparticles and NFs with unfouling and cell-adhesive properties produced by low pressure plasma processes 32
3.1 Introduction 32
3.1.1 Nanofilm embedding silver nanoparticles for antibacterial applications 32
Nanofilm with unfouling cell-adhesive properties in biomedical field produced by PE-CVD 37
3.2 Materials and Methods 40
3.2.2 Reactor setup 41
3.2.3 Experimental Plasma Parameters 42
Characterization of NFs 43
3.3 Results and discussion 44
3.3.1 Study of morphology and chemical properties. 44
3.4 Conclusion 50
4 Low pressure plasma enhanced chemical vapour deposition of chemically different films for biomedical applications 52
4.1 Hydrophobic Teflon like films 54
4.1.1 Introduction 54
4.1.2 Materials and Methods 57
4.1.3 Results and discussion 62
4.2 Films with a different density of COOH on their surface 71
4.2.1 Introduction 71
4.2.2 Materials and Methods 73
4.2.3 Results and discussion 75
4.2.4 Conclusions 90
5 Biological characterization of films produced by plasma processes 92
5.1 Introduction 92
5.1.1Primary cells and Immortal Cell line Cultures 92
5.1.2 In vitro Study of Cells: Evaluation of Adhesion and Viability 97
5.2 Materials and Methods 101
5.2.1 Saos2 cell line 101
5.2.2 Coomassie Blue staning assay 102
5.2.3 MTT test 102
5.3 Results and discussion 104
5.3.1.2. Study of the effect on Saos2 cells of hydrophilic surfaces produced by plasma deposition: effect of CO2 in the feed gas mixture 107
5.4 Conclusion 116
6 Synthesis of Hydroxyapatite Nanoparticles doped with different amount of selenium for tissue engineering applications 117
6.1 Introduction 117
6.1.1 Role of selenium in the biomedical field 117
6.1.2 Synthesis and functionalization of hydroxyapatite nanoparticles 123
6.2 Materials and Methods 130
6.2.1 Synthesis of hydroxyapatite nanoparticles (npHA) 130
6.2.2 Synthesis of hydroxyapatite nanoparticles doped with different percent of selenium (npHA_Se) 131
6.2.3 Chemical-physical characterization of nanoparticles: TEM, SEM, XRD 133
6.2.4 Testing for nanoparticles citotoxicity 135
6.3 Results and discussion 138
6.3.1 Effect of different amount of selenium on morphology, cristalline structure and chemical composition of npHA_Se 138
6.3.2 Effect of hydroxyapatite nanoparticles doped with different percent of selenium on two different cell types: Saos2 and BMSC. 141
6.4 Conclusion 146
References 147
File
| Nome file | Dimensione |
|---|---|
| Tesi_Dan..._2018.pdf | 5.43 Mb |
Contatta l'autore |
|