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Tesi etd-12282017-190420

Type of thesis
Perfezionamento
Author
PIGNATELLI, DANIELA
URN
etd-12282017-190420
Title
Synthesis and functionalization of innovative nanomaterials for biomedical applications
Scientific disciplinary sector
ING-IND/34
Course
INGEGNERIA - Biorobotics
Committee
relatore DARIO, PAOLO
Keywords
  • Free-standing
  • Hydroxyapatite
  • Nanofilms
  • Nanomaterials
  • Nanoparticles
  • Parole chiave: Cells
  • Plasma process
  • Selenium
  • Silver
  • Tissue engineering.
Exam session start date
;
Availability
completa
Abstract
1 Introduction 6<br>1.1 Thesis outline 9<br>2 Free-standing Nanofilms containing molecules of Vancomycin produced by atmospheric pressure plasma process 13<br>2.1 Introduction 13<br>2.1.1 Uses of Vancomycin in biomedical fields 13<br>2.1.2 Atmospheric pressure plasma process to produce bio-composite coating 16<br>2.2 Materials and Methods 21<br>2.2.1 Spin coating deposition parameters to produce sacrificial layer for nanofilm release in water 21<br>2.2.2 Plasma processes reactor setup 23<br>2.2.3 Experimental plasma parameters 24<br>2.2.4 Release of free-standing nanofilms in water 25<br>2.2.5 NFs Characterization (FT-IR, profilometer, SEM analysis, NFs release test) 26<br>2.3 Results and discussion 28<br>2.3.1 Study of delamination, morphology and chemical properties. 28<br>2.3.2 Study of Vancomycin release in water 31<br>2.4 Conclusion 31<br>Chapter 3 32<br>3 NFs embedding silver nanoparticles and NFs with unfouling and cell-adhesive properties produced by low pressure plasma processes 32<br>3.1 Introduction 32<br>3.1.1 Nanofilm embedding silver nanoparticles for antibacterial applications 32<br>Nanofilm with unfouling cell-adhesive properties in biomedical field produced by PE-CVD 37<br>3.2 Materials and Methods 40<br>3.2.2 Reactor setup 41<br>3.2.3 Experimental Plasma Parameters 42<br>Characterization of NFs 43<br>3.3 Results and discussion 44<br>3.3.1 Study of morphology and chemical properties. 44<br>3.4 Conclusion 50<br>4 Low pressure plasma enhanced chemical vapour deposition of chemically different films for biomedical applications 52<br>4.1 Hydrophobic Teflon like films 54<br>4.1.1 Introduction 54<br>4.1.2 Materials and Methods 57<br>4.1.3 Results and discussion 62<br>4.2 Films with a different density of COOH on their surface 71<br>4.2.1 Introduction 71<br>4.2.2 Materials and Methods 73<br>4.2.3 Results and discussion 75<br>4.2.4 Conclusions 90<br>5 Biological characterization of films produced by plasma processes 92<br>5.1 Introduction 92<br>5.1.1Primary cells and Immortal Cell line Cultures 92<br>5.1.2 In vitro Study of Cells: Evaluation of Adhesion and Viability 97<br>5.2 Materials and Methods 101<br>5.2.1 Saos2 cell line 101<br>5.2.2 Coomassie Blue staning assay 102<br>5.2.3 MTT test 102<br>5.3 Results and discussion 104<br>5.3.1.2. Study of the effect on Saos2 cells of hydrophilic surfaces produced by plasma deposition: effect of CO2 in the feed gas mixture 107<br>5.4 Conclusion 116<br>6 Synthesis of Hydroxyapatite Nanoparticles doped with different amount of selenium for tissue engineering applications 117<br>6.1 Introduction 117<br>6.1.1 Role of selenium in the biomedical field 117<br>6.1.2 Synthesis and functionalization of hydroxyapatite nanoparticles 123<br>6.2 Materials and Methods 130<br>6.2.1 Synthesis of hydroxyapatite nanoparticles (npHA) 130<br>6.2.2 Synthesis of hydroxyapatite nanoparticles doped with different percent of selenium (npHA_Se) 131<br>6.2.3 Chemical-physical characterization of nanoparticles: TEM, SEM, XRD 133<br>6.2.4 Testing for nanoparticles citotoxicity 135<br>6.3 Results and discussion 138<br>6.3.1 Effect of different amount of selenium on morphology, cristalline structure and chemical composition of npHA_Se 138<br>6.3.2 Effect of hydroxyapatite nanoparticles doped with different percent of selenium on two different cell types: Saos2 and BMSC. 141<br>6.4 Conclusion 146<br>References 147<br><br>
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